engleski

Moleucar characterisation of B- and T- cell clonality in reactive and malignant lymphoid lesions

Lymphoid cells proliferations, frequently clinically present as persistent lymphadenopathy and occasionally pose serious diagnostic problems in terms of distinguishing benign from malignant processes. In this context molecular analysis of B- and T-cell receptor rearrangements aimed at answering if the process is polyclonal, reactive non-malignant or clonal, accepted as neoplastic, can be useful. We present the results of molecular characterisation of B-cell receptor by PCR based assay for immunoglobulin heavy-chain gene CDR3 region and T-cell receptor by TCRγ multiplex PCR. Materials were 26 fine needle aspirates and 11 samples of paraffin embedded biopsies of lymph nodes from patients with persistent lymphadenopathy and inconclusive morphological and phenotypic diagnosis on the nature of lymphoid proliferation. Molecular findings of clonality were correlated to definitive cytological and histological diagnosis. Of 37 samples 16 were diagnosed as reactive lymphoid proliferations and 21 as malignant process, eg. lymphoma. For cytological samples (N=26) there was a good correlation of clonality with category of reacti...

molecular; clonality

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