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Comparison of ELISA and FEIA method for detecting IgA antibodies to tissue transglutaminase in coeliac disease

Coeliac disease (CD) is a life- long intolerance to alcohol-soluble fraction of gluten. Serology markers used as a screening tool are: antigliadin IgA and IgG antibodies, endomysial IgA antibodies and tissue transglutaminase IgA antibodies which have the highest specificity for CD. The aim of tjis study was to compare two methods for quantitative measurement of IgA antibodies directed to tTG, enzymelinkedimmunosorbent assay (ELISA), Eurospital, Trieste, Italy and fluoroenzymeimmunoassay (FEIA), Pharmacia Diagnostic, Uppsala, Sweden. The study included 47 children who attended Children'hospital Zagreb for suspected CD (M/F=22/25, age 2 months-28 years). Diagnostic specificity, sensitivity, positive and negative predictive values, ROC analysis and correlation coefficient were calculated for both methods. Duodenal biopsy was performed on all patients and was used for accurate diagnosis. Specificity, sensitivity, positive and negative predictive value: Elisa 100% for allparameters, FEIA 100%, 91%, 100%, 93%, respectively, ROC analysis showed higher discriminating power of ELISA (AUC=1.00), versus FEIA (AUC=0.998) at acur off point recommended by anufacturer ; 5.6 AU/ml for ELISA and t.0 IU/ml forFEIA. Correlation coefficient was 0.802. The results included the same specificity and positive predictive value, with higher sensitivity and better negative predictive value of ELISA. ROC analysis showed high discriminating power (ELISA, AUC=1.00 ; FEIA, AUC==.998) for both method and they could be reliablyused in detection of IgA antibodies to tTG:

coeliac disease; ELISA; FEIA

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