Interaction of alcohols with serum LDL. An infrared study (CROSBI ID 117695)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Arrondo, Jose L.R. ; Coto, Xavier ; Milicua, Jose C.G. ; Kveder, Marina ; Pifat, Greta
engleski
Interaction of alcohols with serum LDL. An infrared study
The interaction of low molecular weight alcohols with low density lipoprotein (LDL) has been studied using amide I band-fitting, thermal profiling and two-dimensional infrared correlation spectroscopy (2D-IR). At 0.3 M alcohol, no changes in secondary structure are observed. In the presence of 1 M alcohol, ethanol and propanol decreases protein denaturation temperature and produces changes in the amide I thermal profiles of protein components and in the lipid bands. The 2D-IR synchronous map corresponding to protein or lipid component at 20– 37 °C suggests differences in the presence of propanol. The asynchronous map corresponding to the lipid component indicates changes in bandwidth, compatible with a more fluid environment. In the 37– 80 °C temperature range the thermal profile is different in the presence of propanol, both for the lipid and protein components. The results presented show that when alcohols affect the protein component, the lipid spectrum also varies pointing to an effect on the lipid-protein interaction.
FTIR ; LDL ; pH dependence
rad je rezultat COST D22 medjunarodne suradnje
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
141
2006.
205-215
objavljeno
0009-3084
10.1016/j.chemphyslip.2006.02.013