engleski

Activation of carbonic anhydrase in branchial cavity tissues of lobster Homarus gammarus conditioned by dilute seawater

The activity of carbonic anhydrase CA) and its distribution in the branchial cavity tissues has been studied in the lobster Homarus gammarus from ambient seawater (SW, 38 ppt) and dilute seawater (DSW, 20ppt). In DSW-acclimated lobsters, almost 70% of total CA in tissues of the branchial cavity was found in epipodites and the rest was equally distributed between branchiostegites and gills. In cytosol fractions of tissues from DSW acclimated lobsters, the main proportion of 76% in epipodites, 61% in branchiostegite of total CA activity. Partially purified membranes contained 7% in epipodites and 16% in branchiostegite. These results indicate the importance of epipodite and branchiostegite in CA induction when lobsters are aclimated to DSW.

carbonic anhydrase; dilute seawater; branchial cavitiy tissues; lobster Homarus gammarus

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