engleski

Ethanol influences the hdl oxidation : fluorescence spectroscopy study

Lately, the protective role of high density lipoproteins in atherogenesis is raised again, since the precise molecular mechanism by which HDL prevents atherosclerosis is still uncertain particularly taking into account the oxydation processes. Some clinical investigations are stressing the beneficial effects of moderate alcohol consumption on the cardiovascular diseases incidence. In this study, we have followed by fluorescence spectroscopy the influence of ethanol on the process of HDL oxidation. By measuring the steady state spectra of natural protein fluorophore-tryptophan, the influence of alcohol on apoprotein oxidation has been monitored. The gradual decrease in Trp-fluorescence intensity was less expressed with oxidation. The increase of fluorescence intensity of lipid peroxides was measured to follow the oxidation process in lipid moiety of HDL particle. Membrane molecular probe PRODAN, 6-propionyl-2-dimethil-aminonaphtalene, was used to follow the interactions of apoproteins and lipids during the oxidation process. The increase of ethanol concentration disrupts the communication throught the boundary between protein and lipid monolayer as seen by NRET.

ethanol; HDL; fluorescence

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