Increased activity of Pgp multidrug transporter in patients with Helicobacter pylori infection (CROSBI ID 117451)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Babić, Žarko ; Svoboda-Beusan, Ivna ; Kučišec-Tepeš, Nastja ; Dekaris, Dragan ; Troskot, Rosana
engleski
Increased activity of Pgp multidrug transporter in patients with Helicobacter pylori infection
To determine whether local antibiotic resistance involves P-glycoprotein (Pgp)-mediated active drug outpumping during Helicobacter pylori (H pylori) infection treatment with classic antibiotic therapy. Pgp activity was determined in gastric mucosa biopsy specimens obtained from 53 patients with pathohistologically verified gastritis and microbiologically confirmed H pylori infection, and compared with the Pgp activity in 12 control subjects with normal endoscopic findings. The H pylori positive patients were treated with short-term 7-d therapy consisting of two antibiotics (amoxicillin and azithromycin/metronidazole and clarithromycin) and a proton pump inhibitor. Pgp activity was determined by flow cytometry in the test of rhodamine dye efflux and quantified as mean fluorescence ratio (RMF). Upon the first cycle, H pylori was successfully eradicated in 20 patients, whereas therapy was continued in 33 patients. In the course of antibiotic therapy, RMF increased (P<0.05) and gastric cells showed higher rhodamine dye efflux. The mean pre-treatment RMF values were also higher (P<0.0001) in patients with multiple therapeutic failure than in those with successful H pylori eradication and control subjects. Pgp might be one of the causes of therapy failure in patients with H pylori and antibiotic therapy could be chosen and followed up on the basis of the Pgp transporter local activity.
H pylori ; RMF ; Pgp
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Podaci o izdanju
11 (18)
2005.
2720-2725
objavljeno
1007-9327
2219-2840
10.3748/wjg.v11.i18.2720
Povezanost rada
Kliničke medicinske znanosti, Temeljne medicinske znanosti