engleski

Ethoxyresorufin-O-deethylase induction potency in sediment samples from rivers Lepenica and Morava surrounding area of Kragujevac “ hot spot”

Activation of aryl hydrocarbon receptor (AhR) and induction of CYP1A1 izozyme, whose activity can be determined by measuring activity of 7-ethoxyresorufin-o-deethylase (EROD) in various species and test systems, are well known bioanalyses for assessment of toxicity of persistent toxic compounds (PTCs). Whereas analytical methods determine the concentrations of known substances, bioanalyses with EROD as an end point may also detect the joint activities of nonanalyzed EROD inducing compounds in environmental samples. The main environmental effects of the bombardments (in 1999) of factory Zastava-Kragujevac were damaged transformers which contained Pyralene oil, leaking of several tons of PCBs into the rivers Zdraljica, Lepenica and Morava, and contamination of groundwater by PCBs and heavy metals. Up to 2, 500 kg of oil containing PCBs was released into the environment. Extremely high levels of PCBs were detected in water ecosystems in the area of accident – in the river Lepenica PCB levels were 400-1000mg/l of water. As a consequence of explosions, fires, and PCB combustions, very high levels of PCDDs/Fs were found in soil samples from that area (up to 100, 000ng I-TEQ/kg) few months after bombardments. Very high levels of PCBs and PCDDs/Fs were also found in samples taken around the transformers of the power plant (70-74 g/kg of PCBs and 10, 200 ng I-TEQ/kg of PCDDs/Fs). Right after spillage there was a flood wave from the river Lepenica that caused transport and sedimentation of the pollutants along nearby agricultural area. In 2000, UNEP/UNOPS Clean-up Project YUG 00-R71 was performed for clean up action of environmental damage of hot spots in SCG. However, further investigations which covered hot spot Kragujevac as a zone possibly contaminated with PCBs revealed that there are still ppm concentrations of PCBs as determined by GC/ECD. In this study we explored if these588 contaminants still remained in sediments from rivers Lepenica and Morava. Sediment samples were analyzed by microEROD and GC/ECD analyses.

ethoxyresorufin-O-deethylase; sediment; Lepenica; Morava; Kragujevac

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