Napredna pretraga

Pregled bibliografske jedinice broj: 21490

Equine immune functions following in vivo exposure to Baypamun and the recall antigen tetanus toxoid/toxin


Valpotić, Ivica; Petrinec, Nikica; Šver, Lidija; Tomašković, Marija; Radeljević, Duško
Equine immune functions following in vivo exposure to Baypamun and the recall antigen tetanus toxoid/toxin // Proceedings of the 4th International Meeting "Mechanisms in Local Immunity", Periodicum biologorum 100, Suppl 3 / Vitale, Branko (ur.).
Zagreb: Hrvatsko prirodoslovno društvo, 1998. str. 60-60 (predavanje, međunarodna recenzija, sažetak, znanstveni)


Naslov
Equine immune functions following in vivo exposure to Baypamun and the recall antigen tetanus toxoid/toxin

Autori
Valpotić, Ivica ; Petrinec, Nikica ; Šver, Lidija ; Tomašković, Marija ; Radeljević, Duško

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the 4th International Meeting "Mechanisms in Local Immunity", Periodicum biologorum 100, Suppl 3 / Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 1998, 60-60

Skup
The 4th International Meeting "Mechanisms in local immunity"

Mjesto i datum
Opatija, Hrvatska, 16-19.09.1998

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Immunomodulation; anti-tetanus immunity; horse

Sažetak
We tested whether an immune response modifier (IRM) Baypamun (BPM), given parenterally, could enhance humoral and cellular immune responses in horses selected as tetanus hyperimmune serum donors. The BPM-treated or nontreated horses were repeatedly immunized with tetanus toxoid (TTd) and tetanus toxin (TT) and sampled 9 weeks following the immunization regimens. Phenotypic and functional characteristics of their PBL were evaluate by either flow cytometry or lymphocyte stimulation test using common mitogens (PHA and PWM) and cell-mediated cytolysis against autologous lymphoblasts, respectively. Also, the quantification of serum anti-TTd/TT antibodies and detection of their respective immunoglobulin classes were performed. The PWM-induced proliferation in BPM + TTd/TT-treated horses (SI = 60.4) was about 50% stronger than that of TTd/TT-treated horses (SI = 27.7) before the plasmapheresis. The former horses had more CD2^+ , CD8^+ , ELA II^+, and B-sub.^+ cells, but less CD4^+ cells than the later horses. The PBL from horses that received BPM exhibited stronger cytolytic activity in either DC (+ 37%) or ADCC (+ 57%) than did the cells from horses immunized with TTd/TT only. The titers of antitoxin antibody were much higher (P < 0.01), and the levels of serum IgG and IgG(T) were slightly, but not significantly elevated. The use of BPM, rather as an adjuvant than as an IRM, would be a useful and cost-effective approach for enhancing the specific immune responses of horses previously primed with TTd/TT.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekt / tema
053079

Ustanove
Veterinarski fakultet, Zagreb