engleski

subspicata Vis. growing in Croatia

The present study describes the phytochemical profile and antimicrobial activity of Satureja subspicata Vis. essential oils, collected in Dalmatia (Croatia). Three samples of essential oils were obtained from the aerial parts of the plant by hydrodistillation and analyzed by GC-MS. From the twenty-four compounds representing 97.47% of the oils, carvacrol (16.76%), α -pinene (13.58), p-cymene (10.76%), γ -terpinene (9.54%) and thymol methyl ether (8.83%) appear as the main components. The oils also contained smaller percentages of myrcene, linalool, β -caryophyllene, limonene, geranial acetate, 1-Octen-3-ol, nerol, thymol and borneol. Furthermore, antimicrobial activity of the oil was evaluated using agar diffusion and broth microdilution methods. The antimicrobial test results showed that the oils had a great potential antimicrobial activity against all 13 bacteria and 9 fungal strains. Gram-positive bacteria are more sensitive to the investigated oil, with a range of 0.09 to 6.25 µ ; ; l/ml than Gram-negative bacteria in the range which is significantly higher from 1.56 to 25.00 µ ; ; l/ml. Results presented here may suggest that the essential oil of S. subspicata possess antimicrobial properties, and is therefore a potential source of antimicrobial ingredient in food and pharmaceutical industry.

satureja subspicata; essential oil; antimicrobial activity; GC- MS

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