engleski

In search for genotoxicity: mussels

Marine and freshwater genotoxicity monitoring was conducted as a part of the CROWAT project. Genotoxic burden of the environment was assessed by measuring DNA damage in haemocytes of transplanted (caged) and native mussels: Mytilus galloprovincialis for marine, and Unio pictorum for freshwater ecosystems. The level of DNA damage was evaluated by comet and micronucleus (MN) assays. DNA damage measured by alkaline comet assay appears earlier than MN that requires cell division. At the same time its longevity is to a great extent shorter, indicating recent pollution status, than of micronuclei that lasts as long as the cell itself. Therefore, combined use of these two assays is advantageous because they seem to complement each other. The results of a three year genotoxicity monitoring of Croatian marine and freshwater environment will be presented and discussed.

Genotoxicity; Biomonitoring; Mussels

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