RecBCD Enzyme Overproduction Impairs DNA Repair and Homologous Recombination in Escherichia coli (CROSBI ID 510088)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Đermić, Damir ; Halupecki, Edyta ; Zahradka, Davor ; Petranović, Mirjana
engleski
RecBCD Enzyme Overproduction Impairs DNA Repair and Homologous Recombination in Escherichia coli
The Escherichia coli RecBCD enzyme is a powerful helicase and nuclease that processes DNA molecules containing blunt double-strand DNA end. Mutants deprived of RecBCD enzyme functions are extremely sensitive to DNA-damaging agents, poorly viable and severely deficient in homologous recombination. Remarkably, such important cellular functions rely on only about 10 molecules of RecBCD present in a cell. To determine the effect of an increased concentration of RecBCD enzyme and its derivatives on cellular processes that depend on the enzyme, we introduced wild-type and mutant alleles of recBCD genes on a low-copy-number plasmid into recB and wild-type bacteria and assessed their capacity for DNA repair and homologous recombination. We found that the overproduction of RecBCD enzyme, as well as RecBC and their nuclease-deficient derivatives, impairs both DNA repair and homologous recombination in E. coli. Our collective results suggest that DNA binding and processive helicase activities of the overproduced RecBCD enzyme, or its derivates, impair DNA repair and homologous recombination in E. coli. The cells control these activities of RecBCD by maintaining its extremely low concentration, thereby allowing efficient DNA repair and homologous recombination.
Escherichia coli; RecBCD enzyme; DNA repair; homologous recombination
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Podaci o prilogu
88-88-x.
2005.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts. Second Congress of Croatian Geneticists with International Participation
Franekić Čolić, J. ; Ugarković, Đ.
Zagreb: Hrvatsko genetičko društvo
Podaci o skupu
Second Congress of Croatian Geneticists with international participation.
poster
24.09.2005-27.09.2005
Supetar, Hrvatska