Napredna pretraga

Pregled bibliografske jedinice broj: 211418

The effect of heparin on structural and functional properties of low density lipoproteins


Kriško, Anita; Piantanida, Ivo; Kveder, Marina; Pifat, Greta; Lee, Anthony; Greilberger, Joachim; Kipmen-Korgun, Dijle; Jürgens, Günther
The effect of heparin on structural and functional properties of low density lipoproteins // Biophysical chemistry, 119 (2006), 234-239 doi:10.1016/j.bpc.2005.09.011 (međunarodna recenzija, članak, znanstveni)


Naslov
The effect of heparin on structural and functional properties of low density lipoproteins

Autori
Kriško, Anita ; Piantanida, Ivo ; Kveder, Marina ; Pifat, Greta ; Lee, Anthony ; Greilberger, Joachim ; Kipmen-Korgun, Dijle ; Jürgens, Günther

Izvornik
Biophysical chemistry (0301-4622) 119 (2006); 234-239

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
LDL ; heparin ; fluorescence ; LDL receptor ; binding

Sažetak
Heparin binding to human low density lipoproteins (LDL) and the effect of heparin on the ability of LDL to bind to the LDL receptor has been investigated. Emphasis has been made on the physiological conditions of temperature, pH and the ionic strength. Intrinsic fluorescence spectroscopy of LDL has been applied to follow heparin binding. Fluorescence anisotropy has been measured to describe the changes in apoB and dansyl-heparin dynamics upon binding. Eu3+-labeled LDL binding to the intact LDL receptor has been monitored by time-resolved fluorescence spectroscopy technique. We have found that heparin binds to LDL under the physiological conditions, probably by Van der Waals interactions and hydrogen bonding. Temperature seems to be the most important factor influencing the interaction. Furthermore, the presence of heparin inhibits LDL binding to the intact LDL receptor that might have consequences on the cholesterol metabolism in vivo.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekt / tema
0098037
0098053

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati