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Loss of heterozygosity of NF1 tumour suppressor gene in human sporadic colon cancer


Kapitanović, Sanja; Čačev, Tamara; Radošević, Senka; Spaventi, Radan; Pavelić, Krešimir
Loss of heterozygosity of NF1 tumour suppressor gene in human sporadic colon cancer // Revista de Oncologia 4 Suppl 1 / EACR (ur.).
Granada: Federacion de Sociedades Espanolas de Oncologia y del Instituto Nacional de Cancerologia de Mexico, 2002. (poster, sažetak, znanstveni)


Naslov
Loss of heterozygosity of NF1 tumour suppressor gene in human sporadic colon cancer

Autori
Kapitanović, Sanja ; Čačev, Tamara ; Radošević, Senka ; Spaventi, Radan ; Pavelić, Krešimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Revista de Oncologia 4 Suppl 1 / EACR - Granada : Federacion de Sociedades Espanolas de Oncologia y del Instituto Nacional de Cancerologia de Mexico, 2002

Skup
17th Meeting of the European Association for Cancer Research

Mjesto i datum
Granada, Španjolska, 8-11.6.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Neobjavljeni rad

Ključne riječi
NF1; LOH; colon cancer

Sažetak
Colorectal carcinomas are characterized by multiple genetic aberrations that occur during tumorigenesis. Several tumour suppressor genes associated with colorectal carcinoma have been identified: MCC and APC on chromosome 5q, p53 on chromosome 17p, and DCC and DPC4 on chromosome 18q. We examined 60 cases of human sporadic colon cancer and corresponding normal tissue samples to evaluate LOH at the NF1 gene loci. The purpose of this study was also to evaluate whether LOH at the NF1 gene is associated with clinicopathological characteristics in sporadic colon cancer. DNAs were used for PCR, RFLP, VNTR and LOH analyis. Using 3 polymorphic markers (NF1 exon 5 Rsa I, IVS27AAAT2.1 and IVS38GT53.0) 83, 3% patients were found heterozygous and informative for LOH analysis. DNAs from 18% of tumours exhibited LOH at the NF1 locus. The majority NF1 gene LOH was observed in Dukes' A (56 %), in well defined tumours (43%) and in the tumours that were more than 5 cm in diameter(67%).Our results support the view that malignant progression is a consequence of more than one genetic change and suggest that inactivation of NF1 gene may play a role in the sporadic colon cancer development and progression

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
0098108

Ustanove
Pliva-Istraživački institut,
Institut "Ruđer Bošković", Zagreb