engleski

The servant or the master: the functional characterization of TROL

Our current research is focused on identification of the proteins that participate in regulatory mechanisms of photosynthesis. Although the basic principles of photosynthesis are discovered, the mechanisms that regulate photosynthetic processes are still largely unknown. We are specially interested in rhodaneses, widespread enzymes found in all major evolutionary phyla, that catalyze the transfer of sulfane-sulfur atom from thiosulfate to cyanide. These versatile sulfur carriers have accommodated their function to fulfill the need for sulfane-sulfur atom in distinct metabolic and regulatory pathways. Proposed functions include mobilization of sulfur for iron-sulfur cluster biosynthesis or repair, that might connect rhodaneses and rhodanese-like domains with regulatory roles in anoxygenic photosynthesis. The Arabidopsis thaliana(L.) Heynh. TROL protein is considered to participate in regulation of photosynthesis due to his catalytically inactive rhodanese domain and localization in chloroplasts. By using antisense RNA technique and other methods available, we are trying to elucidate the functional role of the TROL protein.

Arabidopsis thaliana; regulation of photosynthesis; rhodaneses

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