engleski

Association of HLA-DRB1 alleles with severity of sarcoidosis

Sarcoidosis is a multisystemic granulomatous disorder of unknown aetiology, occuring in almost all populations, with prevalence differing in each population on the basis of geographical location and racial differences. Clinical manifestations are characterized by the presence of noncaseating granulomas and numerous immunological abnormalities. It is also associated with an accumulation of CD4+ T cells and macrophages at the inflammatory site. Numerous studies have reported an association of different HLA class II alleles with sarcoidosis in various ethnic groups. Therefore, to identify the significance of HLA-DRB1 alleles in the development of sarcoidosis in the Croatian population, we investigated the distribution of HLA-DRB1 alleles among 90 patients with sarcoidosis and 141 controls. All individuals were typed by PCR-SSP method. Analysis of DRB1 alleles showed significant differences for DRB1*11 and DRB1*0301 allele. DRB1*11 was significantly more frequent (p=0.0051) in patients with chronic onset of disease in comparison with controls, while higher frequency of DRB1*14 (7.0% vs. 4.3%) and lower frequency of DRB1*07 (3.5% vs. 9.6%) were marginally significant. In patients with acute onset of disease DRB1*0301 allele was significantly more frequent (p=0.0021). Among 36 patients with acute (erythema nodosum-EN) or chronic skin sarcoidosis DRB1*0301 allele was also present with statistically significantly higher frequency (p=0.00004) compared with controls. However, patients with no-skin sarcoidosis (N=54) demonstrated higher frequency of DRB1*11 allele (p=0.026). Patients with chest X-ray stage I showed significantly higher presence of DRB1*0301 allele (p=0.00064), while patients with chest X-ray stage II and III showed lower presence of DRB1*11 allele (p=0.0155) in comparison to control group. This study demonstrates that DRB1*11 positive patients have higher risk for chronic form of disease and II or III radiologic stage, while DRB1*0301 positive patients have higher risk for acute form of disease, radiologic stage I and EN.

HLA-DRB1 alleles; PCR; sarcoidosis

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