engleski

QUANTITATIVE ANALYSIS OF PERIOSTEAL CIRCULATION IN LONG BONES

The important role that periosteum plays in healing bone injury and its use as a potential source of osteoprogenitor cells is of high clinical interest. Periosteum is important for bone vascularization and osteogenesis. Periosteal tissue is divided into an outer fibrosis and an inner - active and proliferative layer with intrinsic periosteal blood system. So far few data are available about the quality of periosteal circulation especially regarding different bones and different bone segments. Aim of this study was to quantify microcirculation of inner layer of intact periosteum. We examined periosteum of radius and tibia, taken from 4 sheep 1 to 2 years old. Material for microscopic examination was fixed in 10% neutral buffered formalin, decalcinated in a mixture of formic and sulfuric acid, embedded in paraffin and stained immunohistochemically for CD34 and von Willenbrands factor according standard protocol (DAKO, Denmark). Two samples from each periosteum, 1 cm length respectively, were processed. From each sample 5 fields were analyzed microscopically using a x25 objective and computer-based image analysis system (ISSA, VAMSTECH, Zagreb, Croatia). Periosteal thickness and capillary count were accessed. Capillaries were counted in inner periosteal layer in a determined field of 10 000μ m2. Analysis of intact periosteal microcirculation yielded a mean capillary number of 5, and a mean periosteal thickness of 100mm. Our quantification of the periosteal capillaries indicates no significant differences between periosteum taken from radius and those taken from tibia. From our data we concluded that they represent a basis for studying periosteal capillary circulation during the process of healing or its damage caused by osteosynthesis.

periostal circulation; healing; microcirculation; long bones

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