engleski

Influence of media and temperature on gliotoxin production in Aspergillus fumigatus strains

Gliotoxin is a secondary metabolite of epipolythiodioxopiperazine family with biologically active internal disulfide bridge. It is produced by many fungal species, including Aspergillus fumigatus and A. terreus. Aspegillus fumigatus is a leading causative agent of invasive aspergillosis, which produce gliotoxin, as well as more than twenty other secondary metabolites. Gliotoxin production in situ could have influence on pathogenesis of aspergillosis. In this study we tested the in vitro production of gliotoxin in nine A. fumigatus isolates from the upper respiratory tract of the immunocompromised patients. The effects of media composition and incubation temperature were measured. Gliotoxin was extracted from biomass and its concentration was semiquantitatevely analysed by thin-layer chromatography. Gliotoxin production was higher in yeast-extract liquid medium (YES) that in synthetic Czapek-Dox liquid medium (CZA). Incubation at 37°C have influence on higher gliotoxin production that at 25°C, probably because of expansive mycelar growth on higher temperatures. At concentrations: 4.06 mg/mL (in YES at 37°C) and 1.07 mg/mL (in CZA at 25°C) after three days of incubati, gliotoxin could be detected. YES broth as medium containing 4% sucrose and with 2% of yeast extract represents very rich substrate for the in vitro production of gliotoxin, as one of lipophilic secondary metabolites of A. fumigatus species.

gliotoxin; Aspergillus fumigatus; aspergillosis; Czapek-Dox; yeast-extracts; toxicogenicity

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