engleski

Interleukin-15 produced by decidual adherent cells in vitro enhances perforin, Fas ligand and Granzyme B expression in decidual lymphocytes

NK cells are involved in decidual tissue remodelling events during implantation and early gestation. These cells are endowed with strong cytolytic potential and use either perforin and granzymes or death ligands for exertion of their cytolytic action. There are very few data about the cytokines that regulate their expression. The aim was to investigate whether decidual adherent cells (DAC) and IL-15 affects cytolytic potential of first trimester pregnancy decidual lymphocytes (DL). Decidual mononuclear cells were obtained by enzymatic digestion and density gradient centrifugation. Non adherent DL were collected after 2 hour adherence and cultured with IL-15 (0.5-5 ng/ml), IL-2 (100-1000 u/ml), their combination, DAC at ratio 3:1 and 1:1 or DAC and anti-IL-15 antibody for 18 hours or 72 hours. Perforin, FasL and granzyme B were detected at protein and/or mRNA level in indicated culture conditions. Cytolytic activity of DL against K-562, P815 and 815Fas was measured by 2 hour PKH-26 cytotoxicity assay and the variations of perforin protein and mRNA was measured in DL after a contact with K-562 targets. Interleukin-15 prevented perforin protein down regulation and enhanced mRNAs for perforin, FasL and granzyme B after 18 hour culture like high concentration of IL-2. DAC contain 33.52 ± ; 9.02% of IL-15 expressing cells (HLA-DR+ and HLA.DR-) and sustained perforin expression in DL. Anti-IL-15 antibody abrogated this effect. Interleukin-15, produced by DAC, up regulates cytolytic mediator expression with equal efficiency as high concentration of IL-2.

Interleukin-15; Perforin; Decidual adherent cells; Decidual lymphocytes; Pregnancy

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