engleski

Short term cytolytic mediators expression in decidual lymphocytes is enhanced by IL-15

We investigated whether decidual adherent cells (DAC) and IL-15 affects cytolytic potential of first trimester decidual lymphocytes (DL) in comparison with IL-2. Decidual mononuclear cells were obtained by enzymatic digestion and density gradient centrifugation. Non-adherent DL were collected after 2 hour adherence and cultured with IL-15 (0.5-5 ng/ml), IL-2 (100-1000 U/ml), or both of these cytokines, DAC at ratio 3:1 and 1:1 or DAC and anti-IL-15 antibody for 18 hours or 72 hours. Perforin, FasL and granzyme B were detected at protein and mRNA level in indicated culture conditions. Cytolytic activity of DL against K-562, P815 and P815-Fas was measured by 2 hour PKH-26 cytotoxicity assay and the variations of perforin protein and mRNA were measured in DL after a contact with K-562 targets. Interleukin-15 prevented the perforin protein down-regulation observed after culture of DL and enhanced perforin, FasL and granzyme B transcription after 18 hour-culture. IL-2 had similar effects. DAC contain 33.52 ± 9.02% of IL-15 expressing cells (HLA-DR+ and HLA-DR-) and sustained perforin expression in DL. Anti-IL-15 monoclonal antibody abrogated this effect. Therefore DAC increased cytotoxicity of DL against K-562 mediated by an IL-15 secretion. Interleukin-15, produced by DAC, up-regulates cytolytic mediator expression with equal efficiency as high concentration of IL-2 and perforin mediated cytotoxicity in DL.

interleukin-15; Perforin; decidua

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