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Alkaline comet assayin human biomonitoring of occupational exposure to physical and chemical mutagens


Garaj-Vrhovac, Vera; Kopjar, Nevenka; Želježić, Davor
Alkaline comet assayin human biomonitoring of occupational exposure to physical and chemical mutagens // Central European journal of occupational and environmental medicine, 18 (2003), 1; 265-271 (podatak o recenziji nije dostupan, članak, znanstveni)


Naslov
Alkaline comet assayin human biomonitoring of occupational exposure to physical and chemical mutagens

Autori
Garaj-Vrhovac, Vera ; Kopjar, Nevenka ; Želježić, Davor

Izvornik
Central European journal of occupational and environmental medicine (1219-1221) 18 (2003), 1; 265-271

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Alkaline comet assay; leukocytes; biomonitoring; occupational exposure; ionizing radiation; microwave radiation; antineoplastic drugs; pesticides

Sažetak
The alkaline comet assay was selected as a biomarker of exposure to evaluate the ongoing occupational exposure to physical and chemical mutagens. The primary DNA damage was evaluated by measuring the extent of DNA migration in peripheral blood leukoctes. Parameters of comet assay were studied in 50 medical workers occupationally exposed to ionizing radiation, 10 radar facility workers exposed to microwave radoation, 50 medical workers handling antineoplastic drugs, 10 workers employed in pesticide production, and in 50 corresponding unexposed control subjects. It was found that the subjects who were occupationally exposed to physical and chemical mutagens for various periods of time showed highly significant increase in levels of DNA damage compared to the controls. In some occupationally exposed subjects smoking habit significantly elevated the level of primary DNA damage. The results obtained have confirmed the usefulnedd of the alkaline comet assay as an additional complement to the standard biodosimetric method, reflecting the current level of DNA damage and/or repair in peripheral blood lymphocytes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
0022020

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb