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Pregled bibliografske jedinice broj: 207384

Application of the alkaline comet assay in biodosimetry:Assessment of in vivo DNA damage in human peripheral leukocytes after a gamma radiation incident


Garaj-Vrhovac, Vera; Kopjar, Nevenka; Ražem, Dušan; Vekić, Branko; Miljanić, Saveta; Ranogajec-Komor, Maria
Application of the alkaline comet assay in biodosimetry:Assessment of in vivo DNA damage in human peripheral leukocytes after a gamma radiation incident // Radiation protection dosimetry, 98 (2002), 4; 407-416 (međunarodna recenzija, članak, znanstveni)


Naslov
Application of the alkaline comet assay in biodosimetry:Assessment of in vivo DNA damage in human peripheral leukocytes after a gamma radiation incident

Autori
Garaj-Vrhovac, Vera ; Kopjar, Nevenka ; Ražem, Dušan ; Vekić, Branko ; Miljanić, Saveta ; Ranogajec-Komor, Maria

Izvornik
Radiation protection dosimetry (0144-8420) 98 (2002), 4; 407-416

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cell gel-electrophoresis; white blod cells; ionizing radiation; human lymphocytes; individual cells; base damage; exposure; induction; cancer; repair

Sažetak
The alkaline comet assay was employed in the assessment of DNA damage in leukocytes of a worker incidently exposed to gamma radiation (221mSv, Co-60 source). The comet tail lenghts and tail moments were studied. By using the alkaline comet assay immediately after accidental exposure a high level of DNA damage was recordd one day and one week after the radiation incident. Later on, a decrease in both comet parameters was diminished during a one year period, it was still elevated compared to normal values recorded in leukocytes of a healthy, unexposed person.The results, obtained indicate that the alkaline comet assay is a rapid and sensitive microdosimetric technique and is suitable for in vivo human biomonitoring, especially in cases of incidental exposure to ionizing radiation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
0022020
0098063

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Chemical Abstracts
  • Excerpta Medica
  • The INSPEC Science Abstracts series
  • Quest
  • Energy Information Abstract