engleski

The Problem of Thyroid Antibodies Testing

Nowadays, different methods for determination of thyroglobulin autoantibodies (TGA), microsomal autoantibodies (TMA) and autoantibodies to enzyme thyroid peroxidase (TPO) have been developed. The specificity and sensitivity of these methods depend on the purity of autoantigen preparation itself, valid standardization and type of the methodology used. Although variable in their sensitivity and specificity, these tests are usefuč parameters in clinical practice, especially for patients with autoimmune thyroid disease, patients with differentiated thyroid cancer and pregnant women.Among si six different methods that were used in this study, four methods are based on the EIA principle, and one on the LIA and agglutination methodology each. Comparison of TGA, TMA and TPO values obtained by two, three and four methods in parallel was done on 527 frozen serum samples of outpatients. The concordance of TGA results was found to be in the range from 66% to 83% for two methods and 65 % for three methods. The concordance of TMA/TPO results was in the range from 42% to 100% for two methods and 48% for four methods. The result suggest that the thyroid autoantibody methods need to be standardized and we are not yet certain which one of the methods in most reliable.

Autoantibodies-blood; Thyroglobulin-diagnosis; Thyroglobulin-immunology; Autoantibodies-methods

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