engleski

Analysis of TNF alpha -238, -308, -857 and -1031 promotor polymorphisms in GEP-NET

Neuroendocrine tumors of the gut and pancreas (GEP-NET) produce biogenic amines, peptides and chromogranins, especially chromogranine A (CgA). It has been shown that CgA levels correlate with TNF and soluble TNF receptors, suggesting a regulatory link with this inflammatory cytokine and its role in tumor growth and morphogenesis. TNF alpha gene is mapped to chromosome 6p21.3 and a large number of polymorphisms of its promoter have been described. The aim of our study was to estimate allelic frequency for four promoter SNPs in TNF alpha gene, -238, -308, -857 and -1031, in patients with GEP-NET. DNAs obtained from 30 GEP-NET patients and 150 unrelated healthy volunteers were genotyped for the TNF alpha -238, -308, -857 and -1031 SNPs using real-time PCR TaqMan® ; ; SNP genotyping assays. There was no statistically significant difference in the frequency of alleles associated with altered TNF alpha production between healthy population and patients with GEP-NET. The association between other TNF alpha promoter polymorphism and GEP-NET tumors has to be investigated in the future studies.

GEP-NET; TNF alpha polymorphism

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