Azithromycin potency determination: Optimal conditions for microbiological diffusion method assay (CROSBI ID 82077)
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Turčinov, Tatjana ; Pepeljnjak, Stjepan
engleski
Azithromycin potency determination: Optimal conditions for microbiological diffusion method assay
The research on azithromycin was carried out to determine its potency by the microbiological diffusion method assay on an 8x8 Latin square with two standard and two sample concentrations. Results showed that Sarcina lutea ATCC 9341, Bacillus pumilus NCTC 8241 and Escherichia coli ATCC 10536 reprsented the group recommendable for the analysisof azithromycin by the diffusion method assay. These test microorganisms provided satisfactory precision and accuracy of results tested in 30 successive measurments by 8x8 Latin Square assay. Some pharmacopoeias give official methods for determination of erythromycin potency. The USP recommendation as to the diffusion method on petri dishes and relevant test microorganism is Sarcina lutea ATCC 9341. For potency determination by the diffusion method on 8x8 Latin Square and Petri dishes, PH. Eur. suggest Bacillus pumilus (NCTC 8241, CIP 765,18)and Sarcina lutea ATCC 9341. In the same instances BP suggests Bacillus pumilus NCTC 8241. All the aforesaid recommendations are in agreement with our findings, although our research includes a completly new antibiotic. As azithromycin and erythromycin belong to the same antibiotic group, our results showed that test microorganisms used were similary sensitive to both of them. The recommended group of microorganisms was also supplemented by a new member Escherichia coli ATCC 10536.
Azithromycin; diffusion method assay; 8x8 latin square; potency determination
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