engleski

THE COMPARISON OF RABBIT AND MICE MODEL FOR THE EFFECTIVENESS OF STRONG ADJUVANTS ASSESSMENT

The effectiveness of any novel adjuvant formulation should be preliminary assessed in an animal model. It is generally recommended and widely accepted to use mice (mostly), guinea pig or rabbit model. Among many benefits that mice model offers in comparison to the other two, the most important is the availability of reagents appropriate for the assay of induced immune response. So, investigators usually choose mice for preliminary adjuvant assessment. Here we report on limitations of the mice model when strong adjuvants are investigated. We investigated the effectiveness of two experimental adjuvant formulations containing the immunomodulatory adjuvant of bacterial origin peptidoglycan monomer (PGM). Those two formulations were oil-based formulations Montanide ISA 206 + PGM (206+PGM) and Montanide ISA 720 + PGM (720+PGM). Humoral immune response was followed by determination of antigen-specific IgG. Novel adjuvant formulations were tested in several separate experiments with different antigen types (protein antigens, peptide antigens, snake venom as mixture of antigens) and in each experiment respective antigen specific IgG was quantified and compared to control groups: oil adjuvants (Montanide ISA 206 and ISA 720) themselves, no adjuvant-treated group as negative control and Complete/Incomplete Freund's adjuvant (CFA) treated group as positive control. In mice there was no difference between experimental adjuvant treated groups and respective oil-only treated groups. These results could mislead to conclusion that PGM incorporation didn't give rise to any improvement of the adjuvant formulation. On contrary, in rabbits, the experimental combinations induced statistically significant increase in antigen-specific IgG level, in comparison to respective oil-only treated groups. Interestingly, the CFA treated group in mice resulted in equally high level of antigen-specific IgGs as in all oil-based adjuvants-treated groups. Such findings led us to the conclusion that strong adjuvants completely recruit all mice immune capacities for mounting the immune response, and no differences or improvements could be further discerned.

adjuvant; mouse; rabbit; antigen-specific IgG

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