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Pregled bibliografske jedinice broj: 205071

Assessment of DNA damage in nuclear medicine personnel - comparative study with the alkaline comet assay and the chromosome aberration test


Kopjar, Nevenka; Garaj-Vrhovac, Vera
Assessment of DNA damage in nuclear medicine personnel - comparative study with the alkaline comet assay and the chromosome aberration test // International journal of hygiene and environmental health, 208 (2005), 179-191 (međunarodna recenzija, članak, znanstveni)


Naslov
Assessment of DNA damage in nuclear medicine personnel - comparative study with the alkaline comet assay and the chromosome aberration test

Autori
Kopjar, Nevenka ; Garaj-Vrhovac, Vera

Izvornik
International journal of hygiene and environmental health (1438-4639) 208 (2005); 179-191

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Nuclear medicine; Peripheral blood leukocytes; DNA damage; Alkaline comet assay; Lymphocytes; Chromosome aberration test

Sažetak
Despite much research over the last few decades, there still remains considerable uncertainty as to the genetic impact of ionizing radiation on human populations, particularly at low levels. The aim of the present study was to provide data on the genetic hazards due to occupational exposure of low doses of ionizing radiation in nuclear medicine departments. The assessment of primary DNA damage in peripheral blood leukocytes of medical staff was performed using the alkaline comet assay and the data obtained were compared with the results of conventional cytogenetic biodosimetry using the chromosome aberration (CA) test. Altogether 120 subjects (60 exposed and 60 controls) participated in the study. Statistically signifi cant increases in primary DNA damage and increased frequencies of CAs compared to controls were observed. Within the exposed population, signifi cant inter-individual differences in DNA damage were found, indicating differences in genome sensitivity. Age and gender were not confounding factors, while smoking enhanced the levels of primary DNA damage only in control subjects, as revealed by both biomarkers studied. The present study suggests that genotoxic damage results from exposure to chronic low doses of ionizing radiation in nuclear medicine departments. Therefore, the exposed medical personnel should carefully comply with the radiation protection procedures and should minimize radiation exposure where possible to avoid potential genotoxic effects. The results obtained in this study point to the signifi cance of biological indicators providing information on the actual risk to the radiation exposed individuals. According to our results, the alkaline comet assay and CA test are sensitive biomarkers that can be used as additional complements to physical dosimetry for assessing exposure to radiation in nuclear medicine personnel.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
0022019
0022020

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Biological Abstracts
  • Chemical Abstracts
  • Excerpta Medica
  • Abstracts in Hygiene and Communicable Diseases
  • BIOSIS
  • CAB Abstracts
  • Global Health
  • etc.