engleski

Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11

This study showed that CXCL10 and CXCL11 create a chemokine gradient between the cerebrospinal fluid and serum and recruite CXCR3-expressing T-cells into the CSF of patients with neuroborreliosis. Background: The principal mediators of lymphocyte recruitment into the CSF are chemokines and their receptors. The aim of this study was to evaluate the contribution of chemokine receptor CXCR3 and the corresponding ligands CXCL10 and CXCL11 to the recruitment of peripheral blood (PB) memory CD4+ T-cells into the cerebrospinal fluid (CSF) of patients with acute neuroborreliosis. Methods: This prospective, cross-sectional study included 20 neuroborreliosis patients with early CNS involvement proven by intrathecal synthesis of IgM and IgG antibodies specific for B. burgdorferi antigens and 11 patients with non-inflammatory neurological diseases (NIND). Chemokine receptors on CSF and PB T-cells were analysed by flow cytometry. CXCL10 and CXCL11 in the CSF and serum of patients were quantified by commercially available enzyme-immunoassays. Results: Percentages of memory CD45RO+CD4+ T-cells expressing CXCR3 and CCR5 in the CSF of neuroborreliosis patients were significantly higher compared with the PB. Concentration of CXCL10 in the CSF of neuroborreliosis patients (median 733.3 pg/mL) was significantly increased compared with serum (median 155.4 pg/mL). Concentration of CXCL11 in the CSF of neuroborreliosis patients (median 17.3 pg/mL) was significantly increased compared with serum (median 6.8 pg/mL). CSF of NIND patients contained contained significantly lower concentration of CXCL10 median 9.2 pg/mL) compared to the serum (median 98.5 pg/mL). CXCL11 concentration in the CSF of NIND patients (median 69.8 pg/mL) was significantly increased compared with serum (median 8.9 pg/mL). Conclusions: Our results suggest that CXCL10 and CXCL11 create a chemokine gradient between the CSF and serum and recruite CXCR3-expressing memory CD4+ T-cells into the CSF of neuroborreliosis patients and that CCR5 also plays a role in this process

neuroborreliosis; cytometry

ISSN:1552-4949