engleski

The role of fine-needle aspiration cytology of the testis in men with azoospermija

Aim of the study: Treatment of men with azoospermia is a great diagnostic and therapeutic problem. This study aimed to determine the presence of spermatogenesis in cytological smears of fine needle aspirates of the testes in patients with azoospermia in the evaluation of male infertility. Patients and Methods: Testicular samples were obtained by fine-needle aspiration of both testes in 25 men with azoospermia, as determined by laboratory semen analyses, using 10 mL syringes and 23 G needles. The air-dried smears were stained using May-Grunwald-Giemsa method. The presence of spermatogenetic cells was determined and the number of spermatogonia, primary spermatocytes, spermatids and spermatozoa was counted on 500 Sertoli cells. Results: A satisfactory production of all spermatogenic cells was found in 22 of 50 studied testes (44%) sperm/Sertoli index above 0.10). A reduced, but still present spermatogenesis (including spermatozoa) was found in 10 of 50 testes (20%), and only sertoli cells and no spermatogenetic cells were found in 18 of 50 testes (36%). Conclusion: Testicular fine-needle aspiration is a very important technique for diagnostic evaluation of male infertility. This method enables identification of sperms and planning of assisted reproduction procedures (TESA-ICSI method).

azoospermia; testis; fine-needle aspiration cytology; male infertility

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