engleski

The effect of ochratoxin A on the concentration of protein carbonyls in rats

It was found that the mechanism of the toxicity of ochratoxin A (OTA) involves the production of free radicals and the consequent oxidative stress. Until now the only studied pathway of OTA-caused oxidative damage was peroxidation of lipids. The aim of this study was to check whether OTA induces the production of protein carbonyls, markers of protein oxidation. Rats (5 per group) were given daily OTA (0.5 mg/kg b.w., i.p.) for 7, 14, and 21 days, respectively or solvent only (TRIS) as controls and sacrificed 24 hours after the last treatment. The concentration of OTA in plasma, kidney and liver homogenates increased gradually during the whole length of experiment. The concentration of protein carbonyls in kidney homogenates of OTA treated rats was significantly higher after 14th and 21st treatment than in controls (P<0.05). In liver the concentration of protein carbonyls was significantly higher in OTA treated animals only after 21st treatment (P<0.05). These results confirm that oxidative stress is involved in the mechanism of OTA toxicity, and that it causes the oxidation of proteins.

ochratoxin A; rats; protein carbonyls; oxidative stress

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