engleski

Analysis of several 1, 4-dihydropyridine drugs ba enantioselective liquid chromatography

The chiral resolution of racemic drugs into their enantiomers is of great importance, because the two enantiomers often exhibit different pharmacological activity. This work deals with a group of very popular biologically active 1, 4-dihydropyridine drugs. The medicines from this class of compounds are very often used drugs for treatment of hypertension. They act as calcium antagonists and have the relaxation effect on the vascular smooth muscle cells and cardiac muscle cells. We examined the conditions for separation of 1, 4-dihydropyridine drugs compounds by high performance liquid chromatography on the analytical scale. By changing the chromatographic parameters on a number of most popular columns with chiral stationary phases, we determined optimal conditions for the separations. The analyses for the following substances were performed: isradipine, nitrendipine, nicardipine, nisoldipine, felodipine and amlodipine. The most successful separations for the all investigated compounds, except amlodipine, were achieved on Chiralpak AD column that consist of amylose tris(3, 5-dimethylphenylcarbamat) as chiral selector. This selector was proved as practically universal for chiral recognition of this class of compounds. For amlodipine, which consist the basic primary amine group, the best separation was achieve in the reverse-phase conditions at Ultron ES-OVM column. Validation of methods in selected conditions has shown that the chosen methods are selective and precise with linear response of detector for the all six pairs of enantiomers.

1; 4-dihydropyridines; Chiralpak AD

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