EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats (CROSBI ID 115327)
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Podaci o odgovornosti
Žuvić-Butorac, Marta ; Herak-Kramberger, Carol M. ; Krilov, Dubravka ; Sabolić, Ivan ; Herak, Janko N.
engleski
EPR study of lipid phase in renal cortical membrane organelles from intact and cadmium-intoxicated rats
Numerous studies have demonstrated various structure/function correlations at the level of transport proteins in the kidney cell membranes and various intracellular organelles. However, characterization of the lipid phase of these membranes is rare. Here we report the differences in lipid organization and dynamics of the brush-border membranes (BBM), basolateral membranes (BLM) and endocytotic vesicles (EV), isolated from the kidney cortex of intact rats, studied with the EPR spectroscopy of the spin-labeled membrane lipids. The EPR spectra were analyzed by comparing experimentally observed line shapes with the line shapes calculated according to the theoretical model developed for liquid crystals. In the fitting procedure, three different lipid domains were assumed, which revealed clear differences in the lipid ordering and rotational correlation times, as well as in the lipid partition of these domains in each of the three types of membranes. A similar approach, used to compare the spectroscopic characteristics of BBM from control and cadmium-intoxicated rats, showed significantly changed ordering and increased molecular mobility in the lipid phase of BBM from Cd-treated animals. As tested by an established fluorescence assay, the Cd-induced changes in the lipid mobility co localized with ~5-fold higher conductance
basolateral membrane; brush border membrane; endocytic vesicles; EPR spectroscopy; heavy-metal nephrotoxicity; ion conductance; membrane fluidity
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Podaci o izdanju
Povezanost rada
Fizika, Temeljne medicinske znanosti