engleski

Effects of polysaturated and monosaturated fat diet on insulin action and sensitivity in cultured rat hepatocytes

Introduction: Insulin resistance is a common phenomenon in obesity and Type 2 diabetes. Increased dietary fat intake will lead to impairment of insulin action. The aim of this study was to find out changing of insulin sensitivity in dependence of fat diet and possible direct action of the diet on the liver. Methods: Male Wistar rats were fed ad libitum (3 weeks) with standard, high polyunsaturated fat diet (sunflower oil) and monounsaturated fat diet (olive oil). Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The glucose production and ?-amino isobutyric acid (AIB) transport was measured. Results: Hepatocytes isolated from rats on high polyunsaturated fat diet had an increase in basal and glucagon-stimulated glucose production and insulin had no effects. Insulin significantly decreased and normalized basal glucose production in the liver cells from rats on high monounsaturated fat diet. Insulin-stimulated AIB transport was significantly lower in hepatocytes cultured from rats on high polyunsaturated fat diet, but in hepatocytes from rats on high monounsaturated fat diet was significantly higher than in the control. Conclusion: High polyunsaturated fat diet caused higher glucose production from the liver cells, decrease in insulin action and sensitivity and lead to insulin resistance. High monounsaturated fat diet increased insulin sensitivity in the hepatocytes.

cultured rat hepatocytes; insulin sensitivity; monosaturated fat; polysaturated fat

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