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Dependency of detector response in HPLC-ELSD of carbohydrates on temperature of nebulization and evaporation process and addition of volatile ion-pairing reagents

In this study, ELSD detector response has been evaluated for a series of 15 compounds selected from different groups of carbohydrates (arabinose, cellobiose, erythrose, fructose, galactose, glucose, lactose, melezitose, maltose, mannose, melibiose, rhamnose, sorbose, sucrose and trehalose) as a function of nebulization and evaporation temperature. Additionally, the influence of different volatile ion-pairing mobile phase additives on detection sensitivity was examined.

HPLC; ELSD; detection optimization; carbohydrates; ion-pairing reagents

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