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Pregled bibliografske jedinice broj: 199670

Isolation and purification of a substance immunologically cross-reactive with insulin (SICRI) from tumor tissue


Levanat, Sonja; Pavelić, Krešimir
Isolation and purification of a substance immunologically cross-reactive with insulin (SICRI) from tumor tissue // International journal of biochemistry, 21 (1989), 5; 509-515 doi:10.1016/0020-711X(89)90130-4 (međunarodna recenzija, članak, znanstveni)


Naslov
Isolation and purification of a substance immunologically cross-reactive with insulin (SICRI) from tumor tissue

Autori
Levanat, Sonja ; Pavelić, Krešimir

Izvornik
International journal of biochemistry (0020-711X) 21 (1989), 5; 509-515

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Substance immunologically cross reactive with insulin ; SICRI ; tumor

Sažetak
A substance immunologically cross reactive with insulin (SICRI)was isolated and purified from murine melanoma B16 and myeloid leukemia. Monospecific anti-insulin immunoglobulin C was coupled with CNBr-activated Sepharose 4B to yield an adsorbent. The immunoaffinity column was used to isolate SICRI from tumor tissue. Purified SICRI yielded a single band with mol wt 158 000 on SDS-PAGE. After non-denaturing conditions SICRI appeared again in one single peak. Affinity purified SICRI was shown to be a potent growth factor for different human and murine transformed and normal cells. Biochemical and biological data provide evidence that SICRI and insulin are two distinct biologically active agents.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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