engleski

HPTLC determination of flavonoids and phenolic acids in some Croatian Stachys taxa

Stachys is a large genus belonging to the family Lamiaceae. In common with many other representatives of this family, the aerial parts of the plants belonging to this genus have been used in folk medicine and phytotherapy for many years. The aim of this work was to determine the amounts of biologically active compounds (flavonoids and phenolic acids), present in seven Croatian Stachys taxa (S. alpina, S. officinalis, S. palustris, S. recta subsp. recta, S. recta subsp. subcrenata, S. salviifolia and S. sylvatica). This was achieved by establishing an appropriate extraction procedure and by analyzing the samples by HPTLC with densitometric evaluation. Plant extracts and standard solutions of flavonoids and phenolic acids were applied to silica gel 60 F254 HPTLC plates as 10 mm bands. Plates were developed in vertical glass chambers previously saturated with mobile phase, ethyl acetate-acetic acid-formic acid-water 100+11+11+26 (v/v). After drying and spraying with NP/PEG reagent, the bands were visualized under short and long-wavelength UV light. By use of standard solutions, RF values and fluorescence colors of investigated compounds were determined. Concentrations of the standard solutions were adjusted to the amounts in the samples and again chromatographed with Stachys samples. The Camag Reprostar 3 Videosystem was used for quantitative evaluation. The compound occuring in most of the samples investigated was found to be chlorogenic acid (it was not present in S. recta subsp. recta only) ; isoquercitrin, luteolin 7-O glucoside, rutin, and quercitrin were present in several of the samples. Although S. recta subsp. recta did not contain chlorogenic acid, it was found to be richest in the flavonoids whose presence was determined. This HPTLC method was found to be simple and convenient for rapid screening of active compounds and quantification of the investigated flavonoids and phenolic acids in Stachys taxa.

Stachys; flavonoids; phenolic acids; HPTLC; densitometry

Rad je kao poster prezentiran na skupu Treći hrvatski kongres farmacije s međunarodnim sudjelovanjem, održanom od 27.-30.04.2005.g., Cavtat, Hrvatska ; objavljen u Knjizi sažetaka / Branka Zorc (ur.) ; Zagreb : Farmaceutsko-biokemijski fakultet, 2005. ; str. 187-187 ; ISBN 953-6256-47-9.