engleski

Direct determination of Cd in NaCl containing metallothionein fractions of different red mullet tissues by GF-AAS

Determination of metal concentration in the same fraction of fish tissues in which metallothioneins (MTs) are measured (heat-treated cytosol, S50) is of great importance for correlating MT level with levels of metals that induce it (Zn, Cu, Cd). Separation of MTs from other proteins comprises tenfold dilution of S50 cytosol with 0.9% NaCl solution, and the subsequent heat-treatment. Presence of salt interferes with Cd measurement by graphite furnace AAS. EDTA was, thus, used as a modifier that allows atomisation of Cd at lower temperatures, and consequently efficiently separates Cd signal from NaCl background signal. Method employing atomisation temperature of 900 C, and addition of EDTA solution adjusted to pH  7.0 was validated, showing that reliable Cd measurements are performed in both Cd standard solutions containing 0.9% NaCl, and in the heat-treated cytosol of red mullet tissues, despite the fact that these samples contain a substantial amount of MTs.

cadmium; EDTA; GF-AAS; heat-treated cytosol; metallothioneins; NaCl; red mullet

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