engleski

Changes in catalytic activity of conjugated horse-radish peroxidase

To investigate how different sizes of protein ligands change peroxidase catalytic activity, we labeled IgG and chorionic somatommamotropin (HCS) with horse radish peroxidase (HRP) by two-step glutaraldehyde method. Guiacol and o-phenylendiamidine (OPD) were used as hydrogen donors. Following values were calculated (expressed as conjugated enzyme activity/free enzyme activity): (a)IgG-HRP - Rate-guiacol (Vmax)=0, 23 ; OPD-Rate (Vmax)=0.20 ; compound I formation constant: k1-guicol=0.25 ; k1-OPD=0.22 ; releasing constant k4: guiacol k4=0.23 ; k4-OPD=0.21 ; (b) HCS-HRP conjugate: rate (vmax)guiacol= 0.57 ; Vmax OPD = 0.68 ; compound I formation constant k1-guiacol=0.68 ; k1-OPD=0.75 ; releasing constant k4-guiacol=0.48 ; releasing constant k4-OPD=0.75.

horse radish peroxidase; immunoglobulin; chorionic somatomammotropin; catalytic activity

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