L-ascorbic acid-induced DNA strand breaks and cross links in human neuroblastoma cells (CROSBI ID 114231)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Pavelić, Krešimir
engleski
L-ascorbic acid-induced DNA strand breaks and cross links in human neuroblastoma cells
The effect of high concentrations ofl-ascorbic acid on the in vivo and in vitro growth of human neuroblastoma has been investigated. Directly implemented into cell culture it decreased the DNA, RNA and protein synthesis, and mitosis of neuroblastoma cells, without affecting normal neuronal cells. In vivo treatment of young nude mice bearing human neuroblastoma with 500 mg/kgl-ascorbic acid for the first 10 days markedly inhibited the growth of tumor mass. As determined by alkaline elution, both DNA strand breaks and DNA cross links were observed in tumor cells treated with 1 × 10−4 Ml-ascorbic acid for 2 h. DNA-DNA and DNA-protein cross links in cells treated withl-ascorbic acid were revealed by the proteinase potassium assay. The results indicated thatl-ascorbic acid can be a very effective and selective agent against human neuroblastoma.
L-ascorbic acid ; DNA strand breaks ; cross links ; neuroblastoma cells
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
Povezanost rada
Temeljne medicinske znanosti