engleski

Vagally mediated pentagastrin cytoprotection

The present study investigated the effect of pentagastrin (PG) on absolute alcohol-induced stomach lesions in rats. MATERIAL and METHODS: The experiment was carried out on male Wistar rats, 200-250 g b.w. Gastric mucosal lesions were induced byoral application of absolute alcohol in dose of 5 ml/kg 30 min before animals were pretreated by PG i.p. (1 mg/kg, 250, 100, 1 ug/kg b.w.). The obtained protective effect has been tested by indomethacin (5 mg/kg s.c.) proglumide (400 mg/kg i.p.), domperidone (25 mg/kg i.p.), atropine (1 mg/kg i.p.) and truncal vagotomy. The influence of the applied substances and animal manipulation on gastric juice secretion and its acidity was also measured using a 4h pylorus ligation. The results were teswted using a Student's t-test. RESULTS: PG in a dose of 1 mg/kg showed a strong protective effect on gastric mucosa (P<0.01). Smaller doses (250 and 100 ug/kg) also have a cytoprotective effect (P<0.05). The smallest dose of 1 ug/kg did not show any protection. Cytoprotective effect was not inhibited by indomethacin, proglumide, domperidone and atropine but the truncal vagotomy inhibited that effect. Vagotomy-, but not atropne- induced inhibition clearly implicate involvement of some other, noncholinergic mechanism. The cytoprotective effect of PG was not related to its effect on gastric juice secretion and to its trophic effect.

pentagastrin; cyoprotective effect; gastric mucosal lesions

nije evidentirano