Combined use of liquid chromatography-nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry for the characterization of an acarbose degradation product (CROSBI ID 114042)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Novak, Predrag ; Tepeš, Predrag ; Cindrić, Mario ; Ilijaš, Marina ; Dragojević, Snježana ; Mihaljević, Krešo
engleski
Combined use of liquid chromatography-nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry for the characterization of an acarbose degradation product
Directly coupled LC-MS and LC-NMR were applied to identify and structurally characterize an acarbose degradation product A in acidic media. A comparative analysis of the stop-flow LC-NMR (1H and TOCSY) and LC-MS data provided evidence that A is structurally related to acarbose, differing from the parent compound in a number of subunits present in the molecule. Spectral analysis revealed that A was the alpha-glucosidase inhibitor amylostatin XG. Complementary information obtained from the two methods led to the structural elucidation of A which was later corroborated by high-resolution NMR spectroscopy of the isolated molecule.
nuclear magnetic resonance spectroscopy; acarbose
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Podaci o izdanju
1033 (2)
2004.
299-303
objavljeno
0021-9673
10.1016/j.chroma.2004.01.055
Povezanost rada
Kemija, Farmacija