engleski

Histological assessment of wound healing dynamics in db/db diabetic mice treated with PL 14736

PL 14736 is a peptide under investigation for use in wound healing. We have studied he efficacy of PL 14736, applied daily directly to excisional wounds in genetically obese, insulin resistant (db/db) diabetic mice, which exhibit delayed wound healing. The test substance was compared to the reference substance platelet derived growth factor (PDGF-BB). Female db/db mice each received an 8mm punch biopsy skin wound on the back under anaesthesia. Animals were divided into 3 groups of 25 animals each, treated daily with PDGF-BB, PL 14736 or vehicle (phosphate buffered saline) applied directly to the wound. Five animals per group were each killed on days 6, 9, 12, 18 and 24 post-wounding. Tissue was formalin-fixed, cut through the centre of the wound and embedded in paraffin “ on a side” . Slides were stained with hematoxylin-eosin or van Gieson stain for collagen and elastic fibres. Morphometric evaluation of re-epithelialization was performed together with semi-quantitative granulation tissue analysis. In db/db mice treated with PL 14736, re-epithelialization rate was higher on day 6 after wounding than in wounds treated with vehicle or PDGF-BB (66% versus 44% ; not statistically significant). Maturation of granulation tissue on day 12 post-wounding - seen as formation of collagen bundles - was significantly greater in animals treated with PL 14736 than in those treated with vehicle (p=0.048 in Mann-Whitney U test). PDGF-BB had little effect. We conclude that local treatment with PL 14736 enhances granulation tissue maturation in the first 12 days post-wounding in diabetic mice, while in agreement with the literature, PDGF-BB has a negligible effect on wound healing dynamics in this model.

wound; healing; db/db; PL14736; re-epithelialization; granulation; PDGF-BB

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