engleski

Early-senescing human fibroblasts do not demonstrate accelerated telomere shortening

Most normal mammalian cell lines demonstrate limited growth capacity due to gradual accumulation of senescent cells in the culture. Senescent cells appear in a stochastic manner, initially at a low incidence but with increasing frequency as the culture accumulates more divisions. Since it has been suggested that this phenomenon is regulated by telomere shortening in human cells, we examined telomere length in the subpopulation of senescent cells present in pre-senescent normal human fibroblast cultures. Senescent cells were separated from young cycling cells by either BrdU selection or DiI staining followed by separation on a high-speed cell sorter. Our results demonstrate that telomeres of early-senescing cells shorten at the same rate and are the same length as those of their cycling counterparts.

telomeres; telomerase; abrupt telomere shortening

nije evidentirano