engleski

Loss of heterozygosity of APC and CDH1 genes in laryngeal squamous cell carcinoma

The molecular mechanisms involved in development and progression of laryngeal cancer, specifically squamous cell carcinoma, still need further investigation and elucidation. Twenty-two laryngeal squmous cell carcinomas were analyzed in our study regarding genetic changes of two tumor suppressor genes: Adenomatous polyposis coli (APC) and E-cadherin (CDH1). APC gene instability was tested by polymerase chain reaction/loss of heterozygosity (LOH) using Restriction Fragment Length Polymorphism (RFLP) method. The samples were also screened for mutations by heteroduplex method. E-cadherin gene was analyzed by PCR amplification of tetranucleotide marker (D16S752) linked to E-cadherin gene. The results of our analysis showed 3 samples with LOH of the APC gene out of 15 heterozygous patients (20%). Only one LOH of the CDH1 gene (5.5%) out of 18 heterozygous patients was discovered. D16S752 marker did not reveal any Replication error positive samples. There were 6 samples showing heteroduplexes (33%) encompased in APC's exon 11. Our results suggest that alterations of APC gene may have a role in squamous cell carcinoma development and progression. Detected LOH of the E-cadherin gene indicates that genetic changes of this gene are not very frequent, but that other components of the wnt signalling cascade may also be involved.

APC gene; CDH1 gene; squamous cell carcinoma of the larynx; loss of heterozygosity

nije evidentirano