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Pregled bibliografske jedinice broj: 19488

Methods in citotoxicity assay of atrazine and lindane on primary culture of uterine and ovarian cells and BHK 21 C 13 cells


Gaurina, Višnja; Kniewald, Jasna; Krajač, Ivana; Kniewald, Zlatko
Methods in citotoxicity assay of atrazine and lindane on primary culture of uterine and ovarian cells and BHK 21 C 13 cells // 3. Hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista / Marić, Vladimir i sur. (ur.).
Zagreb: Prehrambeno-biotehnološki fakultet, 1998. str. 144-145 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Methods in citotoxicity assay of atrazine and lindane on primary culture of uterine and ovarian cells and BHK 21 C 13 cells

Autori
Gaurina, Višnja ; Kniewald, Jasna ; Krajač, Ivana ; Kniewald, Zlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3. Hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista / Marić, Vladimir i sur. - Zagreb : Prehrambeno-biotehnološki fakultet, 1998, 144-145

Skup
3. Hrvatski kongres prehrambenih tehnologa, biotehnologa i nutricionista s međunarodnim sudjelovanjem

Mjesto i datum
Zagreb, Hrvatska, 10-12.06.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Pesticides; culture of animal cells; cytotoxicity; reproductive toxicology

Sažetak
Application of pesticides in food production needs control and determination of biodegradation in water, soil and food. The first step in toxicity evaluation and prediction to humans is performed on laboratory animals, usually mices and rats. In order to reduce the number of experimental animals, methods for toxicity assays on animal cell lines and primary cultures are developed. In this work, we used primary cultures of uterine and ovarian cells and BHK 21 C 13 cell line as a test - system for citotoxicity determination of atrazine and lindane. Following parameters are examined: - cell viability and number of cells measured by trypan - blue exclusion method in Fuchs - Rosenthal heamocytometer - total cell protein content measured by Kenacid Blue method - lyzosomal activity measured by Neutral Red method - mitochondrial dehydrogenase activity measured by MTT method 20, 40 and 80 micrograms of atrazine during 24, 48 and 72 h exposure induced cytotoxicity effects in range of 6 - 40 % for uterine cells and 14 - 65 % for BHK 21 C 13 cells respectively. 10, 20 and 30 micrograms of lindane induced citotoxicity effects of 29 - 49 % for uterine cells and 20, 40 and 80 micrograms of lindane induced these effects in range of 24 - 81 % for BHK 21 C 13 cells respectively. These results showed that used methods are fast, simple, reproducible and that can be used in assays also for other xenobiotics in study of reproductive toxicology or teratotoxicity.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija



POVEZANOST RADA


Projekt / tema
058104

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb