engleski

Analysis of VEGF single nucleotide polymorphisms -1154 A/G and 936 C/T in sporadic colon cancer

Colon cancer is one of the most frequently diagnosed cancers in Western Societies. Tumor growth requires the formation of new blood vessels, a process called angiogenesis. The most important regulator of angiogenesis is vascular endothelial growth factor (VEGF) that is overexpressed in several tumors. We examined genotype and allele frequencies of two VEGF SNPs, -1154 A/G in the promotor region and 936 C/T in the 3' untranslated region. VEGF -1154 GG genotype is associated with higher VEGF expression, while -1154 AA genotype is associated with lower VEGF expression. Carriers of a 936-T allele have reduced VEGF plasma levels. VEGF low producers genotypes may confer protection, whereas high producers may have a promoting effect on tumor progression. The aim of this study was to determine wether these SNPs might influence the risk for sporadic colon cancer development and progression. A total 150 colon cancer patients and 150 unrelated cancer-free controls were genotyped for the VEGF -1154 and 936 SNPs using real-time PCR TaqMan® ; ; ; ; SNP genotyping assay and PCR-RFLP method. Genotype frequencies for VEGF -1154 were 12.6%, 49.6% and 37.6% in control population and 14.2%, 49.6% and 36.2% in colon cancer for AA, AG and GG genotype respectively. Genotype frequencies for VEGF 936 were 69.4%, 29.9% and 0.7% in control population and 69.9%, 26.7% and 3.4% in colon cancer for CC, CT and TT genotype respectively. There were no significant associations between colon cancer susceptibility and -1154 and 936 genotypes however a role of other polymorphisms within VEGF cannot be excluded.

VEGF; SNP; sporadic colon cancer

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