engleski

A Fast Immunoradiometric Assay of Human Growth Hormone

Using the components of "Pharmacia" two-site immunoradiometric assay (IRMA) it is possible to determine human growth hormone (hGH) in serum in the time interval od approximately 2 h if the reaction is performed at 37 oC (modified IRMA). The standard IRMA, that is the assay performed at room temperature as recommended by the manufaturer last at least 4 h. Comparison of the results obtained by the standard "Pharmacia" protocol, i.e. modified IRMA vs. standard IRMA, yielded a correlation coefficient of r=0.9996. The results of hGH determinations obtained by two IRMA assays were also compared with the results obtained by standard competitive radioimmunoassay (RIA) of hGH. The following correlation coefficients were obtained: RIA vs. standard IRMA r=0.9997 ; RIA vs. modified IRMA r=0.9989. The modified IRMA procedure has found its value in determination of hGH in serum during and immediately after selective removal of the pituitary (somatotroph) adenoma.

human growth hormone; acromegaly; pituitary adenoma; RIA; IRMA

nije evidentirano