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Pregled bibliografske jedinice broj: 193850

Immunohistochemical testing of a new anti-EGF antibody


Seiwerth, Sven; Guglietta, Antonio; Pecur, Lada; Grabarević, Željko; Đačić, Sanja; Hutinec, Zdenka
Immunohistochemical testing of a new anti-EGF antibody // Folia histochemica et cytobiologica, 34 (1996), 1; 27-29 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 193850 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Immunohistochemical testing of a new anti-EGF antibody

Autori
Seiwerth, Sven ; Guglietta, Antonio ; Pecur, Lada ; Grabarević, Željko ; Đačić, Sanja ; Hutinec, Zdenka

Izvornik
Folia histochemica et cytobiologica (0239-8508) 34 (1996), 1; 27-29

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
EGF - Immunocytochemistry

Sažetak
A new polyclonal anti-EGF antibody, raised in rabbits against a fragment of the humen EGF molecule was evaluated for its usefulness in immunohistochemistry. Various combinations of fixation, concentration, temperature, time and staining procedures were tested on rat submandibulary glands. The best results were obtained using 10% buffered neutral formalin fixation for 24 hours, 1:1000 dilution of primary antibody, incubation time of 60 min with addition of 10% neutral rat serum to the secondary antibody and the ABC staining method. Staining of different human and rat tissues demonstrated strong reactivity in humen parotid glands and both human and rat kidney. Moderate reactivity was found in human and rat gastric, duodenal, colonic as well as buccal mucosa.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina



POVEZANOST RADA


Projekti:
0053019

Ustanove:
Veterinarski fakultet, Zagreb


Citiraj ovu publikaciju

Seiwerth, Sven; Guglietta, Antonio; Pecur, Lada; Grabarević, Željko; Đačić, Sanja; Hutinec, Zdenka
Immunohistochemical testing of a new anti-EGF antibody // Folia histochemica et cytobiologica, 34 (1996), 1; 27-29 (međunarodna recenzija, članak, znanstveni)
Seiwerth, S., Guglietta, A., Pecur, L., Grabarević, Ž., Đačić, S. & Hutinec, Z. (1996) Immunohistochemical testing of a new anti-EGF antibody. Folia histochemica et cytobiologica, 34 (1), 27-29.
@article{article, year = {1996}, pages = {27-29}, keywords = {EGF - Immunocytochemistry}, journal = {Folia histochemica et cytobiologica}, volume = {34}, number = {1}, issn = {0239-8508}, title = {Immunohistochemical testing of a new anti-EGF antibody}, keyword = {EGF - Immunocytochemistry} }
@article{article, year = {1996}, pages = {27-29}, keywords = {EGF - Immunocytochemistry}, journal = {Folia histochemica et cytobiologica}, volume = {34}, number = {1}, issn = {0239-8508}, title = {Immunohistochemical testing of a new anti-EGF antibody}, keyword = {EGF - Immunocytochemistry} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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