Apoptosis of pancreatic B-cells in alloxan treated rats: p53 immunohistochemical study (CROSBI ID 506121)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Veljača, Marija ; Brajša, Karmen ; Glojnarić, Ines ; Ševeljević-Jaran, D. ; Krnić, Žarka ; Ferenčić, Željko
engleski
Apoptosis of pancreatic B-cells in alloxan treated rats: p53 immunohistochemical study
Althrough alloxan has been widely used as an experimental diabetogen, alloxan induced apoptosis of pancreatic B-cells was not described until recently. The aim of this study was to investigate weather p53, tumor suppressor protein, and "the guardian" of genome is involved in apoptosis induction after alloxan challenge. A single sc administration of 150 mg/kg of alloxan monohydrate to female Wistar rats resulted in severe hyperglycemia together with clinical features of diabetes. Histological analysis revealed significant arte of apoptosis in HE-stained, paraffin embedded pancreatic section 48 h after alloxan treatment, in contrast to no apoptosis in control rats. Immunohistochemical staining performed by p53 (Ab-7) kits (Onkogene, Calbiochem), showed significant p53 expression as early as 15 min after alloxan administration, lasting 24 h, no staining in controls. the disapperance of p53 might have been involved in triggering of cell dying by apoptosis in alloxan treated rats.
pancreatic B-cells; apoptosis; p53
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Podaci o prilogu
177-177.
1997.
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
EUROTOX 97 : Diversification in Toxicology : man and environment
poster
25.07.1997-28.07.1997
Århus, Danska