engleski

Confirmation of C. dubliniensis from other Candida spp

Objectives: C. dubliniensis (CD) is often identified as C. albicans (CA), especially in routine mycological practice because two species shares many characteristics: they are "germ-tube" positive and produce chlamydospores. The aim of this study was to distinguish CD from other Candida spp. in immunocompetent and immunodeficient patients' clinical specimens. Methods: All Candida spp. isolates were tested using algorithm-thermotolerance at 45?C, osmotic resistance in broth containing 6.5% of NaCl, and phenotypic features on CHROMagar and Pal-agar. For quality control we used C. albicans ATCC 9028 and C. dubliniensis CBS 7988. Results: One hundred and twenty three strains of Candida spp. isolated from genitourinary tract of immunocompetent patients were tested for CD and was no such strain confirm. In 93 Candida spp. from oral cavity in haematological patients and 10 HIV positive persons we confirm only one strain as CD. CD growth on Sabouraud-agar at 37?C and don't at 45?C, doesn't growth in broth with 6.5% NaCl , on Pal agar colonies have characteristic pseudohyphal roots (with chlamidospores under 400x) . On CHROMagar CD have dark blue-green colour distinguishable from light-blue CA colonies. Conclusions: Using algorithm "germ-tubes" and chamidospore formation together with two methods: growth at 45?C and cultivation on Pal-agar present easy methods for distinguish C. dubliniensis from C. albicans and other Candida spp. in clinical laboratory specimens.

Candida dubliniensis; C. albicans; differentiation; algorithm; yeasts

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