engleski

The role of the SHH/PTCH/SMO pathway in oncogenesis

Constitutive inactivation of the Hedgehog signaling pathway can result in tumorigenesis. Inactivating mutations in the tumor suppressor gene patched (PTCH) have been shown in basal cell carcinoma, medulloblastoma, ovarian fibromas and dermoids. We propose to compare the expression of target genes of hedgehog signaling in basal cell carcinoma with ovarian fibromas and ovarian dermoids to identify tissue specific and common patterns characteristic of tumorigenesis by deregulated Hedgehog signaling. The levels of expression of particular genes of the pathway we determined from extracted RNA which was previously reversally transcribed into cDNA from basal cell carcinomas, ovarian fibromas and dermoids. The levels of expression of some of the pathway genes was enhanced in all cases. This results indicate that the SHH/PTCH/SMO pathway is aberrant in these pathological conditions. Extensions of such analyses will lead to new insights into the mechanism of tumour development and the identification of potential targets and therapies.

signaling pathway; oncogenesis

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